I tried it exactly the way you described (Color 0 is Red in my image, and I am
sure 0 is the index I want.).
Please view the following post to 'Re: Q: Image-Maps in Layered Textures' by Steve
for the original code that does not work.

Thanks,
Hendrik Iben

Bob Hughes wrote:

> You can't get a transparent palette entry which is set in a image program to
> show up as transparent in POV-Ray unless you use the 'transmit' keyword.  Such
> as:
>
> #declare t_image=texture{pigment{
>  image_map {gif "yourimage.gif" transmit 0,1} // index #0, transmit 1
>  }}
>
>  my_texture =
>  texture { t_ground }
>  texture { t_image }
>
> The once keyword is only for non-tiling.  Be certain of your palette index
> number/color relation too.  Black might not always be 0.
>
> Bob

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